I am using an lsrii w facs diva software, 40520bp for indoviolet and 52550 505lp for indoblue. The bd facsvia flow cytometry system is an easytouse benchtop solution that helps meet the challenges of rising costs, decreasing reimbursement, laborintensive manual procedures, fewer resources and higher demands for efficiency, so your laboratory runs smoothly and efficiently. You can change gates and plots, add statistics and. Standardizing application setup across multiple flow. Place a tube with approximately 3 ml of contrad on the sip and leave the sample arm open. Blu products, founded in 2009, designs and manufacturers affordable, mobile devices to suit a wide variety of consumer needs. For information, see formatting density plots in the bd facsdiva software reference manual. Remove the cst beads from the loading port, exit the cst software, and allow the instrument to reconnect to diva. Press the standby button on the control panel if necessary. Bd serves and supports our customers with full offerings beyond our individual products, enabling integrated solutions across healthcare systems to comprehensively improve care and lower costs. This video will show you how to install bd facsdiva 8. See headers and footers on page 100 in the bd facsdiva software reference manual. How to operate the bd facscanto flow cytometer preface dear colleague, the bd facscanto flow cytometer is use to operate, however, it is a delicate instrument for measuring fluorescence of single cells.
Download a free 30 day trial today to quickly analyze your diva data in fcs express. Digital bd cytometers facscanto, facslsrii, facsaria, lsrfortessa, etc. Bd facsdiva software provides one of the most complete and robust feature sets available for flow cytometry. Log in to diva at the prompt with your diva username and password. Standardizing application setup across multiple flow cytometers using bd facsdiva version 6 software bd biosciences march 2012 page 5 compiling data needed to standardize application settings in a laboratory that has multiple sites andor cytometers, a cytometer baseline report needs to be generated for each cytometer. Bd facs lsr fortessa user manual flow cytometry unit biomedical core facility technion faculty of medicine for help please call. The bd facsdiva software reference manual assumes that you have a working. Can i use uncompensated acquisition data of my compensation. This version runs on the windows 7 32bit operating system. Expand the specimen and make the tube active by clicking the arrow next to the tube. Using application settings to standardize flow cytometry.
Based on our training, we were taught how to do manual compensation and how. For general information, see drawing manual gates in the bd facsdiva software reference manual. This video describes the process of manual and auto compensation in bd facsdiva on the lsr ii. Sophie novault bd facs diva software research institut. Amir grau 0542100778 yaakov sakoury 0507498565 ofer shenker 0524568846 a. Becton dickinson pty ltd, 4 research park drive, macquarie university research park, north ryde nsw 21, australia becton dickinson limited, 8 pacific rise, mt. Sep 23, 2019 i am using an lsrii w facs diva software, 40520bp for indoviolet and 52550 505lp for indoblue. Bd biosciences provides flow cytometers, reagents, tools and a wide range of services to support the work of researchers and clinicians who understand disease and improve care. What should i have the scaling factor set to for the ratio of blueviolet fluorescence. Ensure that the center stream is bright, tight, and. Bd facsdiva bead lot files resources software bd biosciences. Flowjos default preferences should display the data the same way as seen on the instrument.
Refill as necessary from large plastic canister containing 1x sheath solution. Download the tutorial pdf and the accompanying data. The program consists of two overlapping parts, which we call views. Contour plots can now be formatted as density plots. Biexponential display is enabled by default, but can be disabled in user preferences, allowing more events to be recorded per experiment. Learn about bd life sciences diagnostic systems qcpi manuals. Exit the cst software and allow the instrument to reconnect to diva. B fcs2csv for comparison with the other two methods. Wellington, auckland, new zealand ec rep bd facsdiva software reference manual for version 8. History revision date change made 341756 rev a 801 production release for bd facsdiva software version 1.
Dec 30, 2017 this video will show you how to install bd facsdiva 8. Plate loader on the dhvi flow facility l01 and l02 benchtop analyzers. Lsr fortessa red switch on the wall behind the monitors fig. Bd facsdiva software reference manual for version 8.
I only came upon this after our bd service engineer sent me a copy of it. Aug 25, 2017 this video describes the process of manual and auto compensation in bd facsdiva on the lsr ii. Compensation of a 7 color panel on the bd lsr ii youtube. Mar 01, 2015 the first, oddly enough, is bd s very own index sorting manual software manual and may not even be installed on your computer or available for download from bd s website. Facs lsrfortessa standard operation protocol hts mode. Mar 31, 2017 download bd facsdiva software by becton, dickinson and company. By default, the plot background color is now set to white. May 17, 20 bd biosciences has developed a protocol for enabling application settings on machines running the facsdiva v6 software, to create settings that allow for standardized consistent fluorescence intensity target values to be obtained across experiments run on different days or instruments. Analog bd cytometers calibur and scan use the cell quest software line, which produce fcs 2. Bd biosciences has developed a protocol for enabling application settings on machines running the facsdiva v6 software, to create settings that allow for standardized consistent fluorescence intensity target values to be obtained across experiments run on different days or instruments. The scheldeman and van zonneveld bioversity international training manual on spatial analysis of plant diversity and distribution. The automated instrument tracking software or bd cytometer setup and tracking also adjusts voltages on pmts so that every preset compensation matrix will be valid if the save settings option is chooses within the software, see tutorials below for more detailed information. Log in to the computer administrator, password bdis and open bd facsdiva software.
Bd facsdiva software is a flexible data acquisition and analysis package. Allow the instrument to connect to the software if necessary. Learn to use bd facsdiva v6 software with our free 6. Disconnect the air line green and fluid line blue from hts mode sheath container. Biexponential scaling in the bd facsdiva software reference manual. The functionality and stability of the software combined with excellent and timely support has made it an important tool for research cytometry at tsri. In high throughput mode, the hts can process a 96well plate in fewer than 15 minutes. See the bd facsdiva software reference manual and the bd cytometer setup and tracking application guide for more details. First, bd has a diva software tutorial you should have gotten a copy with the instrument. The bd facsdiva software reference manual assumes you have a working. Fcs express can load fcs files from the bd facsdiva multicolor flow cytometry. Bd facs canto ii flow cytometer quick user guide v. I make all my users do it on their own time as part of our training protocol. Computer and software login to log into the computer, use lsrii as the user name and lsrii as the password.
Wait 30 minutes for lasers to warm up before running samples. You can change gates and plots, add statistics and create pdf files. To log in to the facsdiva software you need to locate the icon on the desktop and double click it. Bd facsflow sheath solution, or equivalent sheath solution bd facsclean solution or 10% bleach solution 1 part bleach in 9 parts di water r e t a wdi bd lsr ii users guide bd facsdiva software reference manual bd high throughput sampler users guide. In the login window, scroll down through the list of user names to. Close the sample arm and continue running for one minute.
In the login window, scroll down through the list of user names to locate yours. If you are downloading directly to the cytometer workstation, save the zipped bead lot file to the following location. We have facscalibur in the lab with cellquest, but for post acquisition analysis, i would like to use flowjo software. This guide should help you operating the flow cytometer and leaving it such way that another colleague can use it another day. Index sorting from facsdiva to flowjo flow cytometry news. Exports fcs files that can be imported into diva, flowjo or other flow analysis software. This guide contains instructions for using bd facsdiva software version 6. Bd facsuite software minimizes the number of user interactions with the instrument so that the user can spend more time working with samples. Bd facsdiva software features bd facsdiva software bd. If you are the last user for the day, please perform the machine shut down procedure as laid out in section 1. Bd facs lsrfortessa hts operation manual 7 returning to tubemode acquisition verify that the flow cytometer is in standby mode. Biexponential display the biexponential display feature has been enhanced as follows. Setup on page 1 in the bd facsdiva software reference manual.
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